SYCP2 Protein (AA 1-1500) (Strep Tag)

Catalog No. ABIN3137042

Recombinant SYCP2 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for SYCP2 Protein (AA 1-1500) (Strep Tag) (ABIN3137042)

Target: SYCP2 (Synaptonemal Complex Protein 2 (SYCP2))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1500

Purification tag / Conjugate: This SYCP2 protein is labelled with Strep Tag.

Sequence: MPVRPDLQQL EKCIDDALRK NDFKPLLALL QIDICEDVKI KCSKQFLRKL DDLICRELNK KDIQTVSSIL ISIGRCSKNI FILGQAGLQT MIKQGLVQKM VSWFENSKEI ILNQQQSKDE AVMNMIEDLF DLLMVIYDIS DEGKNQVLES FIPQICALVI DSRVNFCIQQ EALKKMNLML DRIPQDANKI LSNQEMLTLM SNMGERILDV GDYELQVGIV EALCRMTTEK RRQELAYEWF SMDFIANAFK EIKDCEFETD CRIFLNLVNG ILGDKRRVYT FPCLSAFLGK YELQIPSDEK LEEFWIDFNL GSHTLSFYIA GDEEDHQWEA VTVPEEKVQM YNIEVRESKK LLTLTLKNIV KISKKEGKEL LFYFDESLEI TNVTKKVFGG NKYKEFTRKQ GISVAKTSIH VLFDASGSQI LVPESQPSPV KENLIHLKEK SDIQKKLVNP LELGNSSSQD EITTPSRKKM SEASMIVPDT DRYTVRSPIL LINTSTPRRS REPLQAINSV EKAVSKTSES GMDYAASPKS RQSDGRKRWN NRANHNKTTA VIQNKQYEDN ESPDQNFNEI EDTLSNVSSA VGKVDKPVLP GVLDISKNTT HSRWACWTPV TTIKLCNNQR SRALPGDTCT QDTGVNKKCT KQKSVSDDDS EETQKGKYSK DVIKCNKSDE AEFCERNIQE QNHPKYSQKK NTANAKKSDW HIESETTYKS VLLNKTTEES LIYKKTCVLS KDVNTTICDK SPSRKSKRNH TKSRKELMSE LTSCELEEIP VRENSKGKRF TGASESLINQ ISRRYNPSDS MMSTRKLKEP QDGSGFSKKP DLQFNKVQRK SYRKLKATVV NVTSECPLDD VYNFSLNGAD EPVIKLGIQE FQATTREASM DNSLKLVKNH DEHDPFLKTK DKRMLSYEKK TLLSDTETEC GCDDSKTDIS WLKEPKTKRL MDYSRNKNTT KYKSRKSRSS MEKGQPRPTM VLNKNSMKND YEVVVDGRTR LPRRATKTKK NYKDLSTSES ESESEKECSY LFKDKLPTKE ETIHSRAQTK KLPEKQQKVF NSEALKGQPS EEQKNSSRLR EGREDSLCLS SASVSRSSSS VEVMRCTEKI TERDFTQDYD YITKSLSPYP KAPSPEFLNG NNSVVGRGQS PRISETSAMC VRKSYSPASG PPFSPRHTPT KNNSVVNMKK ANSVINNQRT QHCNSYSDVS SNSSEKLYME PESPESCDNH MQNKREGNHA ASPLSLSSEK IEKMWFDMPS ENTHVSGPSQ RGSKRRMYLE DDELSNSNEA EVEEAEEREH LLSKKRCQWE NSDQHTFKTS LSTPDFSVPK DWQQELQGAG MFYDNISSDY KRKTDSQHKI MDDFTTKTLK LTQQHLMAMT SQAQGRRDEN VEKFQVTLLD ELEKVEKDSQ TLRDLEKELV DIEEKLVQKM RAYHRCERER FRVLKTSLDK SFLVYNSVYE ESVFTSEMCL MKANMKMLQD KLLKEMHEEE VLNIRRGLQS LFKAHEGNDA
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SYCP2 (Synaptonemal Complex Protein 2 (SYCP2))

Alternative Name: Sycp2

Background: Synaptonemal complex protein 2 (SCP-2) (Synaptonemal complex lateral element protein),FUNCTION: Major component of the axial/lateral elements of synaptonemal complexes (SCS) during meiotic prophase. Plays a role in the assembly of synaptonemal complexes (PubMed:16717126). Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility (PubMed:16717126). Required for insertion of SYCP3 into synaptonemal complexes (PubMed:16717126). May be involved in the organization of chromatin by temporarily binding to DNA scaffold attachment regions. Requires SYCP3, but not SYCP1, in order to be incorporated into the axial/lateral elements. {ECO:0000269|PubMed:11463847, ECO:0000269|PubMed:16717126}.

Molecular Weight: 172.1 kDa

UniProt: Q9CUU3

Pathways: M Phase