JIP3 Protein (AA 1-1337) (Strep Tag)

Catalog No. ABIN3137233

This Recombinant JIP3 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for JIP3 Protein (AA 1-1337) (Strep Tag) (ABIN3137233)

Target: JIP3 (MAPK8IP3) (Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1337

Purification tag / Conjugate: This JIP3 protein is labelled with Strep Tag.

Sequence: MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE RSKMQQVGGS GQTESSLPGR SRKERPTSLN VFPLADGMVR AQMGGKLVPA GDHWHLSDLG QLQSSSSYQC PNDEMSESGQ SSAAATPSTT GTKSNTPTSS VPSAAVTPLN ESLQPLGDYV SVTKNNKQAR EKRNSRNMEV QVTQEMRNVS IGMGSSDEWS DVQDIIDSTP ELDVCPETRL ERTGSSPTQG IVNKAFGINT DSLYHELSTA GSEVIGDVDE GADLLGEFSV RDDFFGMGKE VGNLLLENSQ LLETKNALNV VKNDLIAKVD QLSGEQEVLK GELEAAKQAK VKLENRIKEL EEELKRVKSE AVTARREPRE EVEDVSSYLC TELDKIPMAQ RRRFTRVEMA RVLMERNQYK ERLMELQEAV RWTEMIRASR EHPSVQEKKK STIWQFFSRL FSSSSSPPPA KRSYPSVNIH YKSPTAAGFS QRRSHALCQI SAGSRPLEFF PDDDCTSSAR REQKREQYRQ VREHVRNDDG RLQACGWSLP AKYKQLSPNG GQEDTRMKNV PVPVYCRPLV EKDPSTKLWC AAGVNLSGWK PHEEDSSNGP KPVPGRDPLT CDREGEGEPK STHPSPEKKK AKETPEADAT SSRVWILTST LTTSKVVIID ANQPGTIVDQ FTVCNAHVLC ISSIPAASDS DYPPGEMFLD SDVNPEDSGA DGVLAGITLV GCATRCNVPR SNCSSRGDTP VLDKGQGDVA TTANGKVNPS QSTEEATEAT EVPDPGPSES EATTVRPGPL TEHVFTDPAP TPSSSTQPAS ENGSESNGTI VQPQVEPSGE LSTTTSSAAP TMWLGAQNGW LYVHSAVANW KKCLHSIKLK DSVLSLVHVK GRVLVALADG TLAIFHRGED GQWDLSNYHL MDLGHPHHSI RCMAVVNDRV WCGYKNKVHV IQPKTMQIEK SFDAHPRRES QVRQLAWIGD GVWVSIRLDS TLRLYHAHTH QHLQDVDIEP YVSKMLGTGK LGFSFVRITA LLIAGNRLWV GTGNGVVISI PLTETVVLHR GQLLGLRANK TSPTSGEGTR PGGIIHVYGD DSSDKAASSF IPYCSMAQAQ LCFHGHRDAV KFFVSVPGNV LATLNGSVLD SPSEGPGPAA PAADAEGQKL KNALVLSGGE GYIDFRIGDG EDDETEECAG DVNQTKPSLS KAERSHIIVW QVSYTPE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: JIP3 (MAPK8IP3) (Mitogen-Activated Protein Kinase 8 Interacting Protein 3 (MAPK8IP3))

Alternative Name: Mapk8ip3

Background: C-Jun-amino-terminal kinase-interacting protein 3 (JIP-3) (JNK-interacting protein 3) (JNK MAP kinase scaffold protein 3) (JNK/SAPK-associated protein 1) (JSAP1) (Mitogen-activated protein kinase 8-interacting protein 3) (Sunday driver 2),FUNCTION: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (PubMed:10523642, PubMed:10629060). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner (PubMed:23576431, PubMed:25944905, PubMed:28259553). Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation (PubMed:23576431, PubMed:25944905, PubMed:28259553). Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration (PubMed:23576431, PubMed:25944905, PubMed:28259553). Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (PubMed:23576431, PubMed:25944905, PubMed:28259553). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed:23576431, PubMed:25944905, PubMed:28259553). {ECO:0000269|PubMed:10523642, ECO:0000269|PubMed:10629060, ECO:0000269|PubMed:23576431, ECO:0000269|PubMed:25944905, ECO:0000269|PubMed:28259553}.

Molecular Weight: 147.6 kDa

UniProt: Q9ESN9