IRS4 Protein (AA 1-1216) (Strep Tag)

Catalog No. ABIN3137579

This Recombinant IRS4 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for IRS4 Protein (AA 1-1216) (Strep Tag) (ABIN3137579)

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1216

Purification tag / Conjugate: This IRS4 protein is labelled with Strep Tag.

Sequence: MASCSFSGHQ ALRRLRASAA AAASAALAAV ATTPLLSSGT RTALIGTGSS CPGAMWLSTA TGSRSDSESE EEDLPVGDEV CKRGYLRKQK HGHRRYFVLK LETADAPARL EYYRNARKFR HSVRAAAAAA EAAASGAAVP ALIPPRRVII LYQCFSVSQR ADARYRHLIA LFTQDEYFAM VAENESEQES WYLLLSRLIL ESKRRRCGTL GALPDGEPAA LAAAAAAEPP FYKDVWQVVV KPRGLGHRKE LSGVFRLCLT DEEVVFVRLN TEVASVVVQL LSIRRCGHSE QYFFLEVGRS TVIGPGELWM QVDDSVVAQN MHELFLEKMR ALCADEYRAR CRSYSISIGA HLLTLLSTRR HLGLLPLEPG GWLRRYGLEQ FCRLRAIRER EEMLFTRRFI SPREPPPPFR RGRGHLPRAR RSRRAASVPP SLFRRSAPSP GRIPQPEDVP NDRAREASGS SSGNTEEKDK EGEEGNRGDC IPMNNWGSGN GRGSGGGRGS SGQGSSSQGS GGRQGSGGGQ GSGGQGAGGN QCSGNGQGTA GGHGSGGGGH GSGGGQRPGD GHGSGGGKNS GSGKNSDDGD RGKSVKKRSY FGKFTQSKQQ QTLPPPPPPP PAAGATGGKG KSGGRFRLYF CADRGTKERK EAKEVRDMET SGGATRGPYR ARAFDEDEDD PYVPMRPGVA APLACSSDYM PMAPQNSSAS TKRHSRSPFE DSRGYMMMFP RVSPPPPVPS APKAPDTNKG DDSKDNDSDS DYMFMAPGAG AIPKNPPNAQ GGSSSKSWSS YFSLPSPFQS SPLGQSDHSE YVPMLPGKFL GSGLHKEASF SQGTKNVSSK PSTEASFSKP EDKGSSAKPS DDVPPMNKAK EPNHLSFIAK GTQVKPKPLN PTQERREAAG SRDYINIDFI KRERLVLPSS AQGLPDMRGV VTDPAPTAFS GYLNVEFGVP FPNPTIRLSD LLRVLPGANS IPLAGTRWPF PGSAIGSIVE AGEYIEVIFN PAMTPAMSFA DSAICYDAQT GQIYVVDPFS ECCMDVSLSP GRCSEPPPVA RLRREEAQER RRPQSRSQSL FASTRAAVSA FPTDSLDRDF PAASAVIAAP AEAPLLAVSR ALAVVSALAA APSIGDVFAG FRAAAGVDSA SARGFQPVAG AQAVREFQDL AAGWNPGALN HRARGEDLAA GAAAPPPPPR QIWVLRPQER ADSEDDDDDD DDIYVRMDFA RRDYRK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: IRS4 (Insulin Receptor Substrate 4 (IRS4))

Alternative Name: Irs4

Background: Insulin receptor substrate 4 (IRS-4) (Phosphoprotein of 160 kDa) (pp160),FUNCTION: Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2. {ECO:0000269|PubMed:10403832, ECO:0000269|PubMed:10644546, ECO:0000269|PubMed:11113178, ECO:0000269|PubMed:12618213}.

Molecular Weight: 130.1 kDa

UniProt: Q9Z0Y7