SPAG4 Protein (AA 1-443) (Strep Tag)

Catalog No. ABIN3130139

Recombinant SPAG4 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for SPAG4 Protein (AA 1-443) (Strep Tag) (ABIN3130139)

Target: SPAG4 (Sperm Associated Antigen 4 (SPAG4))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-443

Purification tag / Conjugate: This SPAG4 protein is labelled with Strep Tag.

Sequence: MRRSPRSGSA ASSHNHTPNF YSENSNSSHS ATSGDSNGRR SAGPELGEPE GRRARGSSCG EPALSPGMPG GDTWAGSSRP KLAPRSHNGQ TACGAATVRG GASEPSGSSV VLEEQLNLLP ILDLRQEMPT PRVSKSFLSL LFQVLSMVLS LAVDGLVCVC REICSIRFLF TAVSLLSIFL AALWWGLLYL IPPLENEPTE MLTLSQYHHR VHSQGQQLQQ LQAELNKLHK EVSSVRAAHS ERVAKLVFQR LNEDFVRKPD YALSSVGASI DLEKTSSDYE DQNTAYFWNR LSFWNYARPP SVILEPDVFP GNCWAFEGDK GQVVIRLPGH VQLSDITLQH PPPTVAHTGG ASSAPRDFAV YGLQADDETE VFLGKFIFDV QKSEIQTFHL QNDPPSAFPK VKIQILSNWG HPRFTCLYRV RAHGVRTSEW ADDNATGVTG GPH
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SPAG4 (Sperm Associated Antigen 4 (SPAG4))

Alternative Name: Spag4

Background: Sperm-associated antigen 4 protein (Outer dense fiber-associated protein SPAG4) (SUN domain-containing protein 4),FUNCTION: Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. {ECO:0000269|PubMed:26621829}.

Molecular Weight: 48.6 kDa

UniProt: Q9JJF2