FLVCR Protein (AA 1-560) (Strep Tag)

Catalog No. ABIN3131041

Recombinant FLVCR protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for FLVCR Protein (AA 1-560) (Strep Tag) (ABIN3131041)

Target: FLVCR (FLVCR1) (Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-560

Purification tag / Conjugate: This FLVCR protein is labelled with Strep Tag.

Sequence: MARPDDEVGP AVAPGHPLGK GYLPVPKGAP DGEARLVPQN GPEALNGGPG LGPLIAGAQG GPQALIAAEE ETQARLLPAG DGEDVPCPAC PPRTALSPRR FVVLLIFSLY SLVNAFQWIQ YSSISNVFED FYEVSPLHIN WLSMVYMVAY VPLIFPATWL LDTRGLRLTA LLGSGLNCLG AWVKCGSVQR HLFWVTMLGQ ILCSVAQVFI LGLPSPVASV WFGPKEVSTA CATAVLGNQL GTAVGFLLPP VLVPALGTQN STGLLAHTQN NTDLLAHNIN TMFYGTAFIS TFLFFLTIIA FKEKPPLPPS QAQAVLRDSP PEEYSYKSSI WNLCRNIPFV LLLVSYGIMT GAFYSISTLL NQIILTYYVG EEVNAGRIGL TLVVAGMVGS ILCGLWLDYT KTYKQTTLIV YVLSFIGMLI FTFTLNLGYI IVVFFTGGIL GFFMTGYLPL GFEFAVEITY PESEGMSSGL LNTAAQILGI FFTLAQGKIT TDYNSPEAGN IFLCAWMFVG IILTALIKSD LRRHNINTGL TNIDVKAVPV DSRVDPKPKV MVSIQSESSL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FLVCR (FLVCR1) (Feline Leukemia Virus Subgroup C Cellular Receptor 1 (FLVCR1))

Alternative Name: Flvcr1

Background: Heme transporter FLVCR1 (Feline leukemia virus subgroup C receptor-related protein 1) (Feline leukemia virus subgroup C receptor) (Major facilitator superfamily domain containing 7B) (Mfsd7b),FUNCTION: [Isoform 1]: Heme b transporter that mediates heme efflux from the cytoplasm to the extracellular compartment. Heme export depends on the presence of HPX and is required to maintain intracellular free heme balance, protecting cells from heme toxicity. Heme export provides protection from heme or ferrous iron toxicities in liver, brain, sensory neurons and during erythropoiesis, a process in which heme synthesis intensifies. Possibly export coproporphyrin and protoporphyrin IX, which are both intermediate products in the heme biosynthetic pathway. Does not export bilirubin. The molecular mechanism of heme transport, whether electrogenic, electroneutral or coupled to other ions, remains to be elucidated. {ECO:0000250|UniProtKB:Q9Y5Y0, ECO:0000269|PubMed:18258918, ECO:0000269|PubMed:23187127}., FUNCTION: [Isoform 2]: Heme transporter that promotes heme efflux from the mitochondrion to the cytoplasm. Essential for erythroid differentiation. {ECO:0000269|PubMed:23187127}.

Molecular Weight: 60.5 kDa

UniProt: B2RXV4

Pathways: Transition Metal Ion Homeostasis