C6orf192 Protein (AA 1-459) (Strep Tag)

Catalog No. ABIN3131108

This Recombinant C6orf192 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for C6orf192 Protein (AA 1-459) (Strep Tag) (ABIN3131108)

Target: C6orf192 (Chromosome 6 Open Reading Frame 192 (C6orf192))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-459

Purification tag / Conjugate: This C6orf192 protein is labelled with Strep Tag.

Sequence: MDEAGSPAPA GTGGGDDPGG STRETSRRLS REQIFVLVSA ASMNLGCMMT YSILGPFFPK EAEKKGASNT MIGMIFGCYA LFELLASLVF GKYLVHIGAK FMFIAGMFVS GGVTILFGVL DQLPEGPIFI AMCFLVRIVD AIGFGAAITA SSSILAKAFP NNVATVMGSL EVFSGLGLVA GPPLGGLLYQ SFGYEVPFIF LGCIVLLMIP LNLYILPSYA QESDPGKQSF WKLVTLPKMG LLAFVIISLS SCFGFLDPTL SLFVMEKFSL STGYVGLVFL GLSLSYAISS PLFGLLSDKM PTLRKWLLVF GNLITAGCYM LLGPVPLLHI KSQLWLLVLV LVVNGISAGM SIIPTFPEML SCAYANGFED SISTLGLVSG LFGAMWSVGA FMGPILGGFL CEKIGFEWAA AMQGLWTLLS GVSMALFYLW EDSTARRRSK AQNSLGTEEE RAALLPNDT
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: C6orf192 (Chromosome 6 Open Reading Frame 192 (C6orf192))

Alternative Name: Slc18b1

Background: MFS-type transporter SLC18B1 (Solute carrier family 18 member B1) (Vesicular polyamine transporter) (VPAT),FUNCTION: Proton-coupled polyamine antiporter involved in the translocation of polyamines from cytosol into secretory vesicles prior to their release via exocytosis. Uses the electrochemical proton gradient generated by a V-type proton-pumping ATPase to couple the efflux of protons with the uptake of a polyamine molecule (By similarity). Facilitates vesicular storage of spermine and spermidine in astrocytes with an impact on glutamatergic neuronal transmission and memory formation (PubMed:31800589) (By similarity). Upon antigen stimulation, regulates polyamine accumulation and release in mast cell secretory granules, which in turn potentiates mast cell degranulation and histamine secretion (PubMed:28082679). {ECO:0000250|UniProtKB:D4A9K4, ECO:0000250|UniProtKB:Q6NT16, ECO:0000269|PubMed:28082679, ECO:0000269|PubMed:31800589}.

Molecular Weight: 49.0 kDa

UniProt: D3Z5L6