Protein L Protein (His tag)

Catalog No. ABIN2181671

Recombinant Protein L protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for Protein L Protein (His tag) (ABIN2181671)

Target: Protein L

Protein Type: Recombinant

Origin: Peptostreptococcus magnus

Source: Escherichia coli (E. coli)

Purity: >95 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This Protein L protein is labelled with His tag.

Brand: MABSol®

Characteristics: Recombinant Protein L Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 36-38 kDa polypeptide .Protein L Protein can bind to VL-Kappa.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Buffer: 100 mM NaH2PO4, pH 8.5

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

References

Martinović, Andjelković, Klobučar, Černigoj, Vidič, Lučić, Pavelić, Josić: "Affinity chromatography on monolithic supports for simultaneous and high-throughput isolation of immunoglobulins from human serum." in: Electrophoresis, Vol. 38, Issue 22-23, pp. 2909-2913, (2017) (PubMed).

Target Details

Target: Protein L

Background: Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Gene ID: 17