Protein G Protein (His tag)

Catalog No. ABIN2181669

This Recombinant Protein G protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for Protein G Protein (His tag) (ABIN2181669)

Target: Protein G

Protein Type: Recombinant

Origin: Streptococcus

Source: Escherichia coli (E. coli)

Purity: >98 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This Protein G protein is labelled with His tag.

Brand: MABSol®

Characteristics: Recombinant Protein G Protein is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 31 kDa polypeptide .Protein G Protein can bind to human IgG1 Fc .

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Buffer: 100 mM NaH2PO4, pH 8.5

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

References

Sun, Li, Liang, Zhang, Song, Song, Xue, Wang, Sun, Gu, Li, Li: "A novel immunochromatographic strips assay for rapid and simple detection of systemic lupus erythematosus." in: Scientific reports, Vol. 10, Issue 1, pp. 14178, (2021) (PubMed).

Target Details

Target: Protein G

Background: Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65- kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.