HMGB3 Protein (AA 2-200) (His tag)

Catalog No. ABIN4370752

This Recombinant HMGB3 protein is expressed in Human Cells.

Quick Overview for HMGB3 Protein (AA 2-200) (His tag) (ABIN4370752)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-200

Purification tag / Conjugate: This HMGB3 protein is labelled with His tag.

Purpose: Recombinant Human High Mobility Group Protein B3/HMGB3 (C-6His)

Sequence: MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE VDHHHHHH

Characteristics: Recombinant Human High Mobility Group Protein B3/HMGB3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala2-Glu200) of Human HMGB3 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3

Sub Type: Fusionprotein

Background: High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important role in DNA replication, nucleosome assembly and transcription. HMGB3 binds preferentiallly single-stranded DNA and unwinds double stranded DNA. HMGB3 consists of 200 amino acids and is localized to the cell nucleus. It contains two HMG box DNA-binding domain. HMGB3 binds preferentially single-stranded DNA and unwinds double stranded DNA.
Alternative Names: High Mobility Group Protein B3, High Mobility Group Protein 2a, HMG-2a, High Mobility Group Protein 4, HMG-4, HMGB3, HMG2A, HMG4

Molecular Weight: 24 kDa

UniProt: O15347