S100A8/A9 Complex (Calprotectin) Protein (AA 1-93, AA 2-114) (His tag)
S100A8/A9
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
WB, SDS, ELISA, MS
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- Target See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) Proteins
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-114, AA 1-93
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This S100A8/A9 Complex (Calprotectin) protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human S100A8/S100A9 Protein is produced by our E. coli expression system and the target gene encoding Met1-Glu93 (S100A8) & Thr2-Pro114 (S100A9) is expressed with a 6His tag at the C-terminus.
- Sequence
- MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKEHHHHHH* &TCKMSQLER NIETIINTFH QYSVKLGHPD TLNQGEFKEL VRKDLQNFLK KENKNEKVIE HIMEDLDTNA DKQLSFEEFI MLMARLTWAS HEKMHEGDEG PGHHHKPGLG EGTP*
- Purity
- Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product S100A8/A9 Protein
-
Calprotectin (S100A8/A9) (AA 1-93) protein (His tag)
S100A8/A9 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Calprotectin (S100A8/A9) (AA 1-119) protein (His tag)S100A8/A9 Origin: Chicken Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM HEPES, 10 % Glycerol, 150 mM NaCl, 2.5 mM EDTA, pH 7.4
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
- Alternative Name
- S100A8, S100A9 (S100A8/A9 Products)
- Gene ID
- 6279, 6280
- UniProt
- P05109, P06702
- Pathways
- S100 Proteins
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