PRKG1 Protein (AA 2-686) (His tag)
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- Target See all PRKG1 Proteins
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-686
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This PRKG1 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human PRKG1 Protein is produced by our mammalian expression system and the target gene encoding Gly2-Pro686 is expressed with a 6His tag at the C-terminus.
- Sequence
- MGTLRDLQYA LQEKIEELRQ RDALIDELEL ELDQKDELIQ KLQNELDKYR SVIRPATQQA QKQSASTLQG EPRTKRQAIS AEPTAFDIQD LSHVTLPFYP KSPQSKDLIK EAILDNDFMK NLELSQIQEI VDCMYPVEYG KDSCIIKEGD VGSLVYVMED GKVEVTKEGV KLCTMGPGKV FGELAILYNC TRTATVKTLV NVKLWAIDRQ CFQTIMMRTG LIKHTEYMEF LKSVPTFQSL PEEILSKLAD VLEETHYENG EYIIRQGARG DTFFIISKGT VNVTREDSPS EDPVFLRTLG KGDWFGEKAL QGEDVRTANV IAAEAVTCLV IDRDSFKHLI GGLDDVSNKA YEDAEAKAKY EAEAAFFANL KLSDFNIIDT LGVGGFGRVE LVQLKSEESK TFAMKILKKR HIVDTRQQEH IRSEKQIMQG AHSDFIVRLY RTFKDSKYLY MLMEACLGGE LWTILRDRGS FEDSTTRFYT ACVVEAFAYL HSKGIIYRDL KPENLILDHR GYAKLVDFGF AKKIGFGKKT WTFCGTPEYV APEIILNKGH DISADYWSLG ILMYELLTGS PPFSGPDPMK TYNIILRGID MIEFPKKIAK NAANLIKKLC RDNPSERLGN LKNGVKDIQK HKWFEGFNWE GLRKGTLTPP IIPSVASPTD TSNFDSFPED NDEPPPDDNS GWDIDFVDHH HHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))
- Alternative Name
- PRKG1 (PRKG1 Products)
- Gene ID
- 5592
- UniProt
- Q13976
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