HMGB3 Protein (AA 1-200) (His tag)
Quick Overview for HMGB3 Protein (AA 1-200) (His tag) (ABIN7312412)
Target
See all HMGB3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-200
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Purification tag / Conjugate
- This HMGB3 protein is labelled with His tag.
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Purpose
- Recombinant Human HMGB3 Protein is produced by our mammalian expression system and the target gene encoding Met1-Glu200 is expressed with a 6His tag at the C-terminus.
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Sequence
- MAKGDPKKPK GKMSAYAFFV QTCREEHKKK NPEVPVNFAE FSKKCSERWK TMSGKEKSKF DEMAKADKVR YDREMKDYGP AKGGKKKKDP NAPKRPPSGF FLFCSEFRPK IKSTNPGISI GDVAKKLGEM WNNLNDSEKQ PYITKAAKLK EKYEKDVADY KSKGKFDGAK GPAKVARKKV EEEDEEEEEE EEEEEEEEDE VDHHHHHH
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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High Mobility Group Box 3 (HMGB3) (AA 1-200) protein (His tag)
custom-made HMGB3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
High Mobility Group Box 3 (HMGB3) (AA 1-180) protein (His tag)HMGB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
High Mobility Group Box 3 (HMGB3) (AA 1-200) protein (GST tag)HMGB3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
High Mobility Group Box 3 (HMGB3) (AA 14-176) protein (T7 tag,His tag)HMGB3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, WB, SDS, IP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- HMGB3 (High Mobility Group Box 3 (HMGB3))
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Alternative Name
- HMGB3
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Gene ID
- 3149
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UniProt
- O15347
Target
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