ADIPOQ Protein (His tag)

Catalog No. ABIN5505547

This Recombinant ADIPOQ protein is expressed in Hi-5 Cells.

Quick Overview for ADIPOQ Protein (His tag) (ABIN5505547)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Hi-5 Cells

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This ADIPOQ protein is labelled with His tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human Adiponectin protein expressed in Hi-5 Insect cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 4.0-6.0ug/mL.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Molecular Weight: 35 kDa

NCBI Accession: NP_033735