IL-25 Protein
Quick Overview for IL-25 Protein (ABIN5505694)
Target
See all IL-25 (IL25) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin 25 (Il25 / IL-17E) protein expressed in Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Less than 0.01 ng per μg protein as determined by the LAL method.
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Biological Activity Comment
- Mouse IL-25 binds to recombinant mouse IL-17BR/Fc chimera in a functional ELISA.
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Interleukin 25 (IL25) (AA 33-177) protein (His tag)
IL25 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)IL25 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 33-177) protein (Fc Tag)IL25 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 Origin: Rat Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Interleukin 25 (IL25) proteinIL25 Origin: Human Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Concentration
- > 50 μg/mL
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Buffer
- 20 mM NaHPO4 pH 6.0, 0.5 M NaCl
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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Alternative Name
- Interleukin 25 (Il25 / IL-17E)
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Molecular Weight
- 17.6 kDa
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NCBI Accession
- NP_542767
Target
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