CD112R (AA 28-165) protein (Fc Tag)
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- Target
- CD112R
- Protein Type
- Recombinant
- Protein Characteristics
- AA 28-165
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- Fc Tag
- Application
- SDS-PAGE (SDS)
- Purpose
- CD112R (mouse):Fc (human)(rec.)
- Cross-Reactivity
- Mouse
- Characteristics
- The extracellular domain of mouse CD112R (aa 28-165) is fused at the C-terminus to the Fc portion of human IgG1.
- Purity
- >90 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test).
- Biological Activity Comment
- Binds to the ligand mouse CD112 (PVRL2, Nectin-2) with an EC50 of 5nM.
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.1 mg/mL
- Buffer
- Contains PBS.
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
- Expiry Date
- 6 months
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- Target
- CD112R
- Background
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CD112 Receptor, Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG
CD112 (also called Nectin-2 or poliovirus receptor-related 2 protein) is a type I transmembrane glycoprotein and a member of the Ig gene superfamily (IGSF). CD112/Nectin-2 functions as an adhesion molecule involved in the formation of cell junctions and interactions with other Nectin-family molecules. Nectin-2 is a also a surface marker of some endothelial cells and regulates migration, proliferation and angiogenesis. CD112 functions as an immune checkpoint regulator by modulating T cell signaling, either as a costimulator of T cell function, or as a coinhibitor of T cells proliferation by binding to the receptors CD226 or TIGIT (T cell Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain), respectively. The binding of CD112 ligand to these receptors is weak compared to CD155. Recently, a new receptor of CD112, called CD112R or PVRIG (Poliovirus receptor-related immunoglobulin domain-containing protein) has been characterized, showing high affinity to CD112. CD112R is a new coinhibitory receptor of the PVR family for human T cells, inhibiting TCR-mediated signals upon binding to CD112/Nectin-2. CD112R is preferentially expressed in T lymphocytes and NK cells. Cancer cells evade immune responses as a result of an immunosuppressive tumor microenvironment and CD112R, by playing a role in the regulation of the immune system, could be a new attractive cancer immunotherapy target.
- Molecular Weight
- ~48-50kDa (SDS-PAGE)
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