OspB Protein

Catalog No. ABIN5624576

Supplier Product No.: 000-001-c15

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for OspB Protein (ABIN5624576)

Target: OspB

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: Control Peptide (CP), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Product Details

Supplier Product No.: 000-001-c15

Supplier: Rockland

Purpose: OspB Control Protein

Purification: OspB is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspB and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: OspB is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 72.7 kDa for OspB-MBP, (30.3 kDa for OspB and 42.4 for MBP) in size corresponding to OspB by Western blotting in the appropriate cell lysate or extract. Outer surface protein B was tested in SDS-PAGE and WB.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: OspB

Background:

Synonyms: Outer surface protein B, Borrelia burgdorferi OspB, control protein

Background: OspB, is one of the major Outer Surface Proteins of the outer membrane of Borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF). The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane. They are presumed to play a role in virulence, transmission, or survival in the tick. Two of the major surface Ag of Borrelia burgdorferi, the 31- kDa OspA and 34- kDa OspB proteins, show a high degree of sequence similarity, are encoded by a 49-kb plasmid and share a common promoter, and are coordinately transcribed. OspA, OspB, and OspD are expressed by B. burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals. OspB has a contributing role in the adherence of B. burgdorferi to the tick gut. The C terminus of OspB is important for eliciting a protective immune response to OspB. B. burgdorferi has the ability to vary its surface proteins in response to immune attack. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gene ID: 1194340

NCBI Accession: WP_010890379

UniProt: P17739