Outer Surface Protein C (B. Burgdorferi) (OspC) Protein

Catalog No. ABIN5624577

Supplier Product No.: 000-001-c11

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for Outer Surface Protein C (B. Burgdorferi) (OspC) Protein (ABIN5624577)

Target: Outer Surface Protein C (B. Burgdorferi) (OspC)

Protein Type: Recombinant

Origin: Borrelia burgdorferi

Source: Escherichia coli (E. coli)

Application: ELISA, Western Blotting (WB), Control Peptide (CP), SDS-PAGE (SDS)

Product Details

Supplier Product No.: 000-001-c11

Supplier: Rockland

Purpose: OspC Control Protein

Purification: OspC is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspC and was estimated to be greater than 90% pure.

Sterility: Sterile filtered

Application Details

Application Notes:

Application Note: OspC is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 63.1 kDa for OspC-MBP, (20.7 kDa for OspC and 42.4 for MBP) in size corresponding to OspC by Western blotting in the appropriate cell lysate or extract. Outer Surface Protein C has been tested in SDS-page and western blot.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: Lateral Flow Assay: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: Outer Surface Protein C (B. Burgdorferi) (OspC)

Alternative Name: OspC

Target Type: Bacteria Protein

Background:

Synonyms: OspC, Borrelia burgdorferi OspC, PC, Outer Surface Protein C, control protein

Background: Outer Surface Protein C, or OspC, is a 20.7 kDa immunogenic protein on the outer surface of the spirochete Borrelia burgdorferi. Its function is not known, but it is located with lipid-anchoring sites on the outer cell membrane. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.

Gene ID: 1194415

NCBI Accession: WP_010890595

UniProt: Q07337