VlsE Protein
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- Target
- VlsE
- Protein Type
- Recombinant
- Origin
- Borrelia burgdorferi
- Source
- Escherichia coli (E. coli)
- Application
- ELISA, Western Blotting (WB), Control Peptide (CP), SDS-PAGE (SDS)
- Supplier Product No.
- 000-001-c33
- Supplier
- Rockland
- Purpose
- VlsE Control Protein
- Purification
- VlsE is a fusion protein with an MBP tag and was expressed in E. coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified VlsE and was estimated to be greater than 90% pure.
- Sterility
- Sterile filtered
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- Application Notes
-
Application Note: VlsE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 78.7 kDa for VlsE-MBP, (36.3 kDa for VlsE and 42.4 kDa for MBP) in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract. Variable Lipoprotein Surface-Exposed protein was tested in SDS-page and western blot.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: Lateral Flow Assay: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
- Expiry Date
- 6 months
-
- Target
- VlsE
- Background
-
Synonyms: Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein, control protein
Background: Variable Lipoprotein Surface-Exposed protein, or VlsE, is a lipoprotein on the surface of the Lyme Disease spirochete Borrelia burgdorferi, detectable during all its life stages. It can exist as many different isoforms. VlsE has variable regions (VRs) and invariable regions (IRs). Some IRs are anchored in the outer membrane of the bacteria and some are antigens exposed on the membrane surface. Replacement of the VR by Borrelia within days of being transferred to a mammalian host presents new surface antigens to the host immune system, and helps Borrelia avoid a strong reaction by host immune systems. The VlsE is apparently not modified as much in the tick or in the rodent vector, when compared to in the mammal host. Several putative envelope proteins of B. burgdorferi appear to be expressed only in the infected mammalian host. The VRs are antigenic, irregularly shaped loops on the bacterial surface which may help to hide both membrane-incorporated and surface portions of adjacent proteins from immune cells. These VR loops are coded by antigenic cassettes. The protein loops can therefore be switched in or out of the protein, or different type loops traded. In B. burgdorferi there seem to be at least fifteen different VlsE cassettes that can insert into any of the variable regions of VlsE, allowing it to appear as millions of different antigens. Similar, but smaller, systems also operate for OSP-A, OSP-B, OSP-C, and other proteins. Some current research involves determination of control of cassette activation. One IR region, C6, of the VlsE protein, consistently stimulates a strong immune response. Its presentation may be a decoy that misdirects the immune system from less protected sites by causing competition for binding antibodies. The bound antibodies are thus not available for binding important therapeutic proteins. This may help Borrelia to enter T-cells, leading to their destruction. Because IR6 is invariable and found in all life stages of B. burgdorferi, it has been used in an ELISA diagnostic test for early IgM of Lyme Disease. Lyme disease proteins are ideal for researchers interested in immunology, neurology, rheumatology, coinfections, autoimmune, and neurodegenerative diseases.
- Gene ID
- 1194357
- UniProt
- Q5DVG3
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