EEF2 Protein (AA 2-858) (His tag)

Catalog No. ABIN5709437

This Recombinant EEF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for EEF2 Protein (AA 2-858) (His tag) (ABIN5709437)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 2-858

Purification tag / Conjugate: This EEF2 protein is labelled with His tag.

Sequence: VNFTVDQIRA IMDKKANIRN MSVIAHVDHG KSTLTDSLVC KAGIIASARA GETRFTDTRK DEQERCITIK STAISLFYEL SENDLNFIKQ SKDGAGFLIN LIDSPGHVDF SSEVTAALRV TDGALVVVDC VSGVCVQTET VLRQAIAERI KPVLMMNKMD RALLELQLEP EELYQTFQRI VENVNVIIST YGEGESGPMG NIMIDPVLGT VGFGSGLHGW AFTLKQFAEM YVAKFAAKGE GQLGPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLDSEDKDKE GKPLLKAVMR RWLPAGDALL QMITIHLPSP VTAQKYRCEL LYEGPPDDEA AMGIKSCDPK GPLMMYISKM VPTSDKGRFY AFGRVFSGLV STGLKVRIMG PNYTPGKKED LYLKPIQRTI LMMGRYVEPI EDVPCGNIVG LVGVDQFLVK TGTITTFEHA HNMRVMKFSV SPVVRVAVEA KNPADLPKLV EGLKRLAKSD PMVQCIIEES GEHIIAGAGE LHLEICLKDL EEDHACIPIK KSDPVVSYRE TVSEESNVLC LSKSPNKHNR LYMKARPFPD GLAEDIDKGE VSARQELKQR ARYLAEKYEW DVAEARKIWC FGPDGTGPNI LTDITKGVQY LNEIKDSVVA GFQWATKEGA LCEENMRGVR FDVHDVTLHA DAIHRGGGQI IPTARRCLYA SVLTAQPRLM EPIYLVEIQC PEQVVGGIYG VLNRKRGHVF EESQVAGTPM FVVKAYLPVN ESFGFTADLR SNTGGQAFPQ CVFDHWQILP GDPFDNSSRP SQVVAETRKR KGLKEGIPAL DNFLDKL

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EF2

Background: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movent of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Molecular Weight: 99.2 kDa

UniProt: P13639

Pathways: AMPK Signaling