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Histone H3.3 Protein (AA 2-136) (His-SUMO Tag)

Recombinant Histone H3.3 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN5709639

Quick Overview for Histone H3.3 Protein (AA 2-136) (His-SUMO Tag) (ABIN5709639)

Target

See all Histone H3.3 (H3F3A) Proteins
Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Protein Type

Recombinant

Origin

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Human

Source

  • 9
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Escherichia coli (E. coli)

Application

SDS-PAGE (SDS)

Purity

> 90 %
  • Protein Characteristics

    AA 2-136

    Purification tag / Conjugate

    This Histone H3.3 protein is labelled with His-SUMO Tag.

    Sequence

    ARTKQTARKS TGGKAPRKQL ATKAARKSAP STGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSAAIG ALQEASEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA

    Purification

    SDS-PAGE
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.1-2 mg/mL

    Buffer

    20 mM Tris-HCl based buffer, pH 8.0

    Storage

    -80 °C,4 °C,-20 °C

    Storage Comment

    Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Target

    Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

    Alternative Name

    H33

    Background

    Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacent throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a tplate. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome rodeling.

    Molecular Weight

    31.2 kDa

    UniProt

    P84243
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