Histone H3.3 Protein (AA 2-136) (His-SUMO Tag)
Quick Overview for Histone H3.3 Protein (AA 2-136) (His-SUMO Tag) (ABIN5709639)
Target
See all Histone H3.3 (H3F3A) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 2-136
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Purification tag / Conjugate
- This Histone H3.3 protein is labelled with His-SUMO Tag.
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Sequence
- ARTKQTARKS TGGKAPRKQL ATKAARKSAP STGGVKKPHR YRPGTVALRE IRRYQKSTEL LIRKLPFQRL VREIAQDFKT DLRFQSAAIG ALQEASEAYL VGLFEDTNLC AIHAKRVTIM PKDIQLARRI RGERA
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Purification
- SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1-2 mg/mL
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Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
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Storage
- -80 °C,4 °C,-20 °C
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Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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Alternative Name
- H33
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Background
- Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacent throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a tplate. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome rodeling.
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Molecular Weight
- 31.2 kDa
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UniProt
- P84243
Target
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