NDST1 Protein (AA 40-556, Isoform 2) (His-SUMO Tag)
Quick Overview for NDST1 Protein (AA 40-556, Isoform 2) (His-SUMO Tag) (ABIN5709995)
Target
See all NDST1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 40-556, Isoform 2
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Purification tag / Conjugate
- This NDST1 protein is labelled with His-SUMO Tag.
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Sequence
- GWKRGLEPSA DAPEPDCGDP PPVAPSRLLP LKPVQAATPS RTDPLVLVFV ESLYSQLGQE VVAILESSRF KYRTEIAPGK GDMPTLTDKG RGRFALIIYE NILKYVNLDA WNRELLDKYC VAYGVGIIGF FKANENSLLS AQLKGFPLFL HSNLGLKDCS INPKSPLLYV TRPSEVEKGV LPGEDWTVFQ SNHSTYEPVL LAKTRSSESI PHLGADAGLH AALHATVVQD LGLHDGIQRV LFGNNLNFWL HKLVFVDAVA FLTGKRLSLP LDRYILVDID DIFVGKEGTR MKVEDVKALF DTQNELRAHI PNFTFNLGYS GKFFQTGTNA EDAGDDLLLS YVKEFWWFPH MWSHMQPHLF HNQSVLAEQM ALNKKFAVEH GIPTDMGYAV APHHSGVYPV HVQLYEAWKQ VWSIRVTSTE EYPHLKPARY RRGFIHNGIM VLPRQTCGLF THTIFYNEYP GGSSELDKII NGGELFLTVL LNPVSAPQPM AAGEKGLLHS LSAADTGFLE PGKGGEA
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Purification
- SDS-PAGE
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N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (His tag)
custom-made NDST1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (His tag)custom-made NDST1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) (AA 1-882) protein (GST tag)NDST1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1) protein (Myc-DYKDDDDK Tag)NDST1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.1-2 mg/mL
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Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
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Storage
- -80 °C,4 °C,-20 °C
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Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- NDST1 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 1 (NDST1))
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Alternative Name
- NDST1
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Background
- Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.
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Molecular Weight
- 73.5 kDa
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UniProt
- P52848
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Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Glycosaminoglycan Metabolic Process
Target
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