TOX1 (AA 35-269) protein (His tag)

Catalog No. ABIN5711000

This Recombinant protein is produced in Escherichia coli (E. coli).

Quick Overview for TOX1 (AA 35-269) protein (His tag) (ABIN5711000)

Target: TOX1

Protein Type: Recombinant

Origin: Bordetella pertussis

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 35-269

Purification tag / Conjugate: His tag

Sequence: DDPPATVYRY DSRPPEDVFQ NGFTAWGNND NVLDHLTGRS CQVGSSNSAF VSTSSSRRYT EVYLEHRMQE AVEAERAGRG TGHFIGYIYE VRADNNFYGA ASSYFEYVDT YGDNAGRILA GALATYQSEY LAHRRIPPEN IRRVTRVYHN GITGETTTTE YSNARYVSQQ TRANPNPYTS RRSVASIVGT LVRMAPVIGA CMARQAESSE AMAAWSERAG EAMVLVYYES IAYSF

Purification: SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: TOX1

Background: S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD+ into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their mbrane receptors.

Molecular Weight: 30.3 kDa

UniProt: P04977