VL2 (AA 1-462), (full length) protein (His tag)

Catalog No. ABIN5714172

Product Details

Target: VL2

Protein Type: Recombinant

Protein Characteristics: full length, AA 1-462

Origin: Human Papillomavirus 18 (HPV-18)

Source: Yeast

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Sequence: MVSHRAARRK RASVTDLYKT CKQSGTCPPD VVPKVEGTTL ADKILQWSSL GIFLGGLGIG TGSGTGGRTG YIPLGGRSNT VVDVGPTRPP VVIEPVGPTD PSIVTLIEDS SVVTSGAPRP TFTGTSGFDI TSAGTTTPAV LDITPSSTSV SISTTNFTNP AFSDPSIIEV PQTGEVAGNV FVGTPTSGTH GYEEIPLQTF ASSGTGEEPI SSTPLPTVRR VAGPRLYSRA YQQVSVANPE FLTRPSSLIT YDNPAFEPVD TTLTFDPRSD VPDSDFMDII RLHRPALTSR RGTVRFSRLG QRATMFTRSG TQIGARVHFY HDISPIAPSP EYIELQPLVS ATEDNDLFDI YADDMDPAVP VPSRSTTSFA FFKYSPTISS ASSYSNVTVP LTSSWDVPVY TGPDITLPST TSVWPIVSPT APASTQYIGI HGTHYYLWPL YYFIPKKRKR VPYFFADGFV AA

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: VL2

Target Type: Viral Protein

Background: Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, escorts the genomic DNA into the nucleus, in particular by promoting virion endosomal escape. It is involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assbly, encapsidates the genome by direct interaction with the viral DNA .

Molecular Weight: 51.6 kDa

UniProt: P06793