IL1F9 Protein (AA 1-169) (His tag)

Catalog No. ABIN5853123

Recombinant IL1F9 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL1F9 Protein (AA 1-169) (His tag) (ABIN5853123)

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-169

Purification tag / Conjugate: This IL1F9 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMRGTPGD ADGGGRAVYQ SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL1F9 (Interleukin 1 Family Member 9 (IL1F9))

Alternative Name: IL36G

Background: IL36G is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Recombinant human IL36B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 21.1 kDa (192aa), confirmed by MALDI-TOF

NCBI Accession: NP_062564

UniProt: Q9NZH8