HERC5 Protein (AA 681-1024) (His tag)
Quick Overview for HERC5 Protein (AA 681-1024) (His tag) (ABIN5853692)
Target
See all HERC5 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 681-1024
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Purification tag / Conjugate
- This HERC5 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG
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Hect Domain and RLD 5 (HERC5) (AA 1-1024) protein (His tag)
custom-made HERC5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hect Domain and RLD 5 (HERC5) (AA 681-1024) protein (His-IF2DI Tag)HERC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Hect Domain and RLD 5 (HERC5) (AA 915-1024) protein (GST tag)HERC5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Denatured
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- HERC5 (Hect Domain and RLD 5 (HERC5))
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Alternative Name
- HERC5
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Background
- HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E.coli.
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Molecular Weight
- 43 kDa (367aa)
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NCBI Accession
- NP_057407
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UniProt
- Q9UII4
Target
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