ATP1B3 Protein (AA 57-279) (His tag)

Catalog No. ABIN5853699

This Recombinant ATP1B3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ATP1B3 Protein (AA 57-279) (His tag) (ABIN5853699)

Target: ATP1B3 (ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 80% by SDS-PAGE

Product Details

Protein Characteristics: AA 57-279

Purification tag / Conjugate: This ATP1B3 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.15M NaCl, 1  mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ATP1B3 (ATPase, Na+/K+ Transporting, beta 3 Polypeptide (ATP1B3))

Alternative Name: ATP1B3

Background: ATP1B3 belongs to the X (+) /potassium ATPases subunit beta family. This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Recombinant human ATP1B3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 27.4 kDa (246aa) confirmed by MALDI-TOF

NCBI Accession: NP_001670

UniProt: P54709

Pathways: Thyroid Hormone Synthesis