Caspase 2 Protein (AA 348-452) (His tag)

Catalog No. ABIN5853713

Recombinant Caspase 2 protein expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 2 Protein (AA 348-452) (His tag) (ABIN5853713)

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 80 % by SDS - PAGE

Product Details

Protein Characteristics: AA 348-452

Purification tag / Conjugate: This Caspase 2 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MAGKEKLPKM RLPTRSDMIC GYACLKGTAA MRNTKRGSWY IEALAQVFSE RACDMHVADM LVKVNALIKD REGYAPGTEF HRCKEMSEYC STLCRHLYLF PGHPPT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Denatured

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.4M urea, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Alternative Name: CASP2

Background: CASP2 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. Recombinant human CASP2 protein, fused to His-tag at N-terminus, was expressed in E.coli .

Molecular Weight: 14.1kDa (126aa)

NCBI Accession: NP_116764

UniProt: P42575

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway