GFPT1 Protein (AA 332-699) (His tag)

Catalog No. ABIN5853831

This Recombinant GFPT1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for GFPT1 Protein (AA 332-699) (His tag) (ABIN5853831)

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS - PAGE

Product Details

Protein Characteristics: AA 332-699

Purification tag / Conjugate: This GFPT1 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MGSQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GVATRQVLEE LTELPVMVEL ASDFLDRNTP VFRDDVCFFL SQSGETADTL MGLRYCKERG ALTVGITNTV GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 50 % glycerol, 2  mM DTT, 2  mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))

Alternative Name: GFPT1

Background: GFPT1 is the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. Recombinant human GFPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 43.7kDa(391aa) confirmed by MALDI-TOF

NCBI Accession: NP_001231639

UniProt: Q06210

Pathways: ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process