IdnK Protein (AA 1-187) (His tag)
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- Target See all IdnK (IDNK) Proteins
- IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-187
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Origin
- E. coli
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IdnK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product IDNK Protein
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IdnK Gluconokinase Homolog (IDNK) (AA 1-187) protein (His tag)
custom-made IDNK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
IdnK Gluconokinase Homolog (IDNK) (AA 1-184) protein (His tag)custom-made IDNK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
IdnK Gluconokinase Homolog (IDNK) (AA 1-141) protein (GST tag)IDNK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
IdnK Gluconokinase Homolog (IDNK) (AA 1-187) protein (His tag)IDNK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))
- Alternative Name
- idnk (IDNK Products)
- Background
- D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.4 kDa (210aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_418689
- UniProt
- P39208
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