GSTA1 Protein (AA 1-223) (His tag)
Quick Overview for GSTA1 Protein (AA 1-223) (His tag) (ABIN5854233)
Target
See all GSTA1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-223
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Purification tag / Conjugate
- This GSTA1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ
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Biological Activity Comment
- Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
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Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-223) protein (His tag)
custom-made GSTA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) protein (His tag)custom-made GSTA1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) protein (GST tag)GSTA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) (Active) proteinGSTA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS Active
Glutathione S-Transferase alpha 1 (GSTA1) (AA 1-222) proteinGSTA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid, In Phosphate buffered saline ( pH 7.4) containing 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))
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Alternative Name
- Gsta1
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Background
- Gsta1 also known as Glutathione S-transferase, A1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant mouse Gsta1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 28kDa (246aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_032207
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UniProt
- P13745
Target
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