CA12 Protein (AA 25-301) (His tag)

Catalog No. ABIN5854284

Recombinant CA12 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for CA12 Protein (AA 25-301) (His tag) (ABIN5854284)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 25-301

Purification tag / Conjugate: This CA12 protein is labelled with His tag.

Sequence: APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLS

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In phosphate buffered saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: Carbonic Anhydrase 12/CA12

Background: CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 31.94 kDa (283aa)

NCBI Accession: NP_001209

UniProt: O43570