CD99 Protein (CD99) (AA 23-122) (hIgG-His-tag)
Quick Overview for CD99 Protein (CD99) (AA 23-122) (hIgG-His-tag) (ABIN5854927)
Target
See all CD99 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 23-122
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Purification tag / Conjugate
- This CD99 protein is labelled with hIgG-His-tag.
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Sequence
- ADPDGGFDLS DALPDNENKK PTAIPKKPSA GDDFDLGDAV VDGENDDPRP PNPPKPMPNP NPNHPSSSGS FSDADLADGV SGGEGKGGSD GGGSHRKEGE EADLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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CD99 (CD99) (AA 23-122) protein (Fc Tag)
CD99 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD99 (CD99) (AA 23-122) protein (His tag)CD99 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD99 (CD99) (AA 25-122) protein (His-IF2DI Tag)CD99 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
CD99 (CD99) (AA 1-185) protein (GST tag)CD99 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD99 (CD99) protein (Fc Tag)CD99 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
CD99 (CD99) protein (Fc Tag)CD99 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- CD99
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Alternative Name
- CD99
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Background
- CD99, also known as CD99 antigen isoform a, is a type 1 transmembrane glycoprotein and the founding member of the CD99 family of molecules. Cells known to express this protein include fibroblasts, neutrophils, T cells, CD34+ stem cells, monocytes and endothelial cells. Also, it plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Recombinant human CD99, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 37.3 kDa (342aa)
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NCBI Accession
- NP_002405
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UniProt
- P14209
Target
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