SPR Protein (AA 1-262) (His tag)
Quick Overview for SPR Protein (AA 1-262) (His tag) (ABIN5854975)
Target
See all SPR ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-262
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Purification tag / Conjugate
- This SPR protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQ KDTFQSGAHV DFYDC
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Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag)
custom-made SPR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag)custom-made SPR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Sepiapterin Reductase (SPR) (AA 1-261) protein (His-IF2DI Tag)SPR Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Sepiapterin Reductase (SPR) (AA 1-261) protein (GST tag)SPR Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sepiapterin Reductase (SPR) (AA 1-261) protein (His tag)SPR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.5) containing 1 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- SPR (Sepiapterin Reductase (SPR))
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Alternative Name
- Spr
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Background
- Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
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Molecular Weight
- 30.3kDa (285aa), confirmed by MALDI-TOF
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NCBI Accession
- NP_035597
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Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
Target
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