MCAM Protein (AA 24-559) (His tag)
Quick Overview for MCAM Protein (AA 24-559) (His tag) (ABIN5855057)
Target
See all MCAM ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 24-559
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Purification tag / Conjugate
- This MCAM protein is labelled with His tag.
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Sequence
- VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGAAAL EHHHHHH
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Melanoma Cell Adhesion Molecule (MCAM) (AA 24-559) protein (His tag)
MCAM Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-563) protein (His tag)MCAM Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) protein (GST tag)MCAM Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Melanoma Cell Adhesion Molecule (MCAM) (AA 24-646) protein (His-DYKDDDDK-Strep II Tag)MCAM Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Alternative Name
- CD146/MCAM
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Background
- MCAM, also known as cell surface glycoprotein MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is associated with various carcinomas such as tumor progression, metastasis and may be involved in embryonic neural development. This protein also plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Recombinant human MCAM protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 61kDa (547aa)
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NCBI Accession
- NP_006491
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UniProt
- P43121
Target
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