Biglycan Protein (BGN) (AA 38-369) (hIgG-His-tag)

Catalog No. ABIN5855132

This Recombinant Biglycan protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Biglycan Protein (BGN) (AA 38-369) (hIgG-His-tag) (ABIN5855132)

Target: Biglycan (BGN)

Protein Type: Recombinant

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 38-369

Purification tag / Conjugate: This Biglycan protein is labelled with hIgG-His-tag.

Sequence: ADPDEEASGS DTTSGVPDLD SVTPTFSAMC PFGCHCHLRV VQCSDLGLKT VPKEISPDTT LLDLQNNDIS ELRKDDFKGL QHLYALVLVN NKISKIHEKA FSPLRKLQKL YISKNHLVEI PPNLPSSLVE LRIHDNRIRK VPKGVFSGLR NMNCIEMGGN PLENSGFEPG AFDGLKLNYL RISEAKLTGI PKDLPETLNE LHLDHNKIQA IELEDLLRYS KLYRLGLGHN QIRMIENGSL SFLPTLRELH LDNNKLSRVP AGLPDLKLLQ VVYLHSNNIT KVGINDFCPM GFGVKRAYYN GISLFNNPVP YWEVQPATFR CVTDRLAIQF GNYKKLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Biglycan (BGN)

Alternative Name: Biglycan

Background: Bgn, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP). It binds to the growth factors BMP-4 and influences BMP-4 bioactivity. It plays an important role in stabilizing fibrotic scars after experimental myocardial infarction. It may function in connective tissue metabolism by binding to collagen fibrils and TGF-beta and may promote neuronal survival. Its ablation improves cardiac function and attenuates left ventricular hypertrophy and fibrosis after long-term pressure overload. It is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. Recombinant mouse Bgn, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 64.6 kDa (574aa)

NCBI Accession: NP_031568

UniProt: P28653

Pathways: Glycosaminoglycan Metabolic Process