Peroxiredoxin 5 Protein (PRDX5) (AA 53-214)

Catalog No. ABIN7281359

Recombinant Peroxiredoxin 5 protein expressed in Escherichia coli (E. coli).

Quick Overview for Peroxiredoxin 5 Protein (PRDX5) (AA 53-214) (ABIN7281359)

Target: Peroxiredoxin 5 (PRDX5)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % by SDS-PAGE

Product Details

Protein Characteristics: AA 53-214

Sequence: MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is >9,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM HEPES (  pH 7.4)

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Peroxiredoxin 5 (PRDX5)

Alternative Name: PRDX5

Background: Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that PRDX5 is involved in intracellular redox signaling. Recombinant human PRDX5, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 17kDa (162aa) confirmed by MALDI-TOF

NCBI Accession: NP_036226

UniProt: P30044

Pathways: Cell RedoxHomeostasis