SETD8 Protein (AA 195-352) (His tag)
Quick Overview for SETD8 Protein (AA 195-352) (His tag) (ABIN5855179)
Target
See all SETD8 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 195-352
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Purification tag / Conjugate
- This SETD8 protein is labelled with His tag.
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Sequence
- MKAELQSEER KRIDELIESG KEEGMKIDLI DGKGRGVIAT KQFSRGDFVV EYHGDLIEIT DAKKREALYA QDPSTGCYMY YFQYLSKTYC VDATRETNRL GRLINHSKCG NCQTKLHDID GVPHLILIAS RDIAAGEELL YDYGDRSKAS IEAHPWLKHH HHHHH
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 1-349) protein (His tag)
custom-made SETD8 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 1-393) protein (His tag)custom-made SETD8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 195-352) protein (His tag)SETD8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 5 mM DTT, 0.2M NaCl, 1 mM EDTA, 50 % glycerol.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))
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Alternative Name
- KMT5A
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Background
- KMT5A, also known as N-lysine methyltransferase KMT5A isoform 1, is an enzyme which catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1). It is implicated in cell-cycle-dependent transcriptional silencing and mitotic regulation in metazoans. It acts as a barrier to prevent cellular senescence through chromatin-mediated regulation of senescence-associated metabolic remodeling. It mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. The loss of KMT5A concurrently stimulate nucleolar function and retinoblastoma protein-mediated mitochondrial metabolism. Recombinant human KMT5A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 18.9 kDa (165aa)
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NCBI Accession
- NP_065115
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UniProt
- Q9NQR1
Target
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