Osteopontin Protein (AA 17-287) (His tag)

Catalog No. ABIN6239742

This Osteopontin protein is produced in Escherichia coli (E. coli).

Quick Overview for Osteopontin Protein (AA 17-287) (His tag) (ABIN6239742)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 17-287

Purification tag / Conjugate: This Osteopontin protein is labelled with His tag.

Characteristics: Tag location: N-terminal His Tag

Biological Activity Comment: Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml, 200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1. Sample O.D. value Corrected Concentration of MCP-1(cell supernatant of MCF-7 cells) (ng/mL) stimulated with OPN (100ng/mL) 0.970 0.922 1.72 stimulated with OPN (200ng/mL) 1.155 1.107 2.11 unstimulated 0.674 0.626 1.11 Table 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN Figure 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN.

Application Details

Application Notes: Isoelectric Point: 4.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin (OPN)

Background: Alternative Names: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin

Molecular Weight: 60kDa

UniProt: P10451

Pathways: Regulation of Cell Size