CXCL2 Protein (AA 32-100) (His tag,GST tag)

Catalog No. ABIN6239880

This CXCL2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CXCL2 Protein (AA 32-100) (His tag,GST tag) (ABIN6239880)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 32-100

Purification tag / Conjugate: This CXCL2 protein is labelled with His tag,GST tag.

Characteristics: Tag location: N-terminal His and GST Tag

Biological Activity Comment: Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GROb with DPP4.

Application Details

Application Notes: Isoelectric Point: 6.7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: Growth Regulated Oncogene Beta (GROb)

Background: Alternative Names: CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2

Molecular Weight: 41kDa

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin