CAPNL1 Protein (AA 75-356) (His tag)
Quick Overview for CAPNL1 Protein (AA 75-356) (His tag) (ABIN6239882)
Target
See all CAPNL1 ProteinsBiological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 75-356
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Purification tag / Conjugate
- This CAPNL1 protein is labelled with His tag.
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Characteristics
- Tag location: N-terminal His Tag
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Biological Activity Comment
- Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.
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Application Notes
- Isoelectric Point: 5.3
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
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Alternative Name
- Calpain 1, Large Subunit (CAPN1)
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Background
- Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain
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Molecular Weight
- 33kDa
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UniProt
- P97571
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Pathways
- Apoptosis
Target
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