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CAPNL1 Protein (AA 75-356) (His tag)

CAPNL1 protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN6239882

Quick Overview for CAPNL1 Protein (AA 75-356) (His tag) (ABIN6239882)

Target

See all CAPNL1 Proteins
CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Biological Activity

Active

Origin

  • 2
  • 2
  • 1
  • 1
Rat

Source

  • 4
  • 2
Escherichia coli (E. coli)

Application

Activity Assay (AcA), Cell Culture (CC)

Purity

> 95 %
  • Protein Characteristics

    AA 75-356

    Purification tag / Conjugate

    This CAPNL1 protein is labelled with His tag.

    Characteristics

    Tag location: N-terminal His Tag

    Biological Activity Comment

    Calpain 1, Large Subunit (CAPN1) is an intracellular protease that requires calcium for its catalytic activity. Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has broad endopeptidase specificity. Besides, Signal Transducer And Activator Of Transcription 3 (STAT3) has been identified as an interactor of CAPN1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CAPN1 and recombinant rat STAT3. Briefly, CAPN1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAT3-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CAPN1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CAPN1 and STAT3 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CAPN1 with STAT3.
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  • Application Notes

    Isoelectric Point: 5.3

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

    Preservative

    Dithiothreitol (DTT), Other preservative, ProClin

    Precaution of Use

    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target

    CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

    Alternative Name

    Calpain 1, Large Subunit (CAPN1)

    Background

    Alternative Names: PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain

    Molecular Weight

    33kDa

    UniProt

    P97571

    Pathways

    Apoptosis
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