RHOD Protein (AA 18-207) (His tag)

Catalog No. ABIN6386731

Recombinant RHOD protein expressed in Escherichia coli (E. coli).

Quick Overview for RHOD Protein (AA 18-207) (His tag) (ABIN6386731)

Target: RHOD (Ras Homolog Family Member D (RHOD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 18-207

Purification tag / Conjugate: This RHOD protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C

Characteristics: RhoD, 18-207aa, Human, His tag, E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA, 0.1mM PMSF

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: RHOD (Ras Homolog Family Member D (RHOD))

Alternative Name: RhoD

Background: Rho-related GTP-binding protein, also known as RhoD, belongs to the small GTPase superfamily. The small GTPase Rho D contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. Recombinant human RhoD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 23.8 kDa (211aa) confirmed by MALDI-TOF

NCBI Accession: NP_055393