PSMB10 Protein (AA 40-273) (His tag)

Catalog No. ABIN7279563

Product Details

Target: PSMB10 (Proteasome (Prosome, Macropain) Subunit, beta Type 10 (PSMB10))

Protein Type: Recombinant

Protein Characteristics: AA 40-273

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PSMB10 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: TTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE

Characteristics: PSMB10, 40-273aa, Human, His tag, E.coli

Purity: > 85% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PSMB10 (Proteasome (Prosome, Macropain) Subunit, beta Type 10 (PSMB10))

Alternative Name: PSMB10/MECL1 (PSMB10 Products)

Background: PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Expression of the PSMB10 gene is induced by gamma interferon, and PSMB10 replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 26.9 kDa (255aa) confirmed by MALDI-TOF

NCBI Accession: NP_002792

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA