L-Lactate Dehydrogenase (LLDD) (AA 1-396) protein (His tag)
LLDD
Origin: E. coli
Host: Escherichia coli (E. coli)
Recombinant
> 90% by SDS-PAGE
SDS
1 image
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- Target
- L-Lactate Dehydrogenase (LLDD)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-396
- Origin
- E. coli
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMIISAA SDYRAAAQRI LPPFLFHYMD GGAYSEYTLR RNVEDLSEVA LRQRILKNMS DLSLETTLFN EKLSMPVALA PVGLCGMYAR RGEVQAAKAA DAHGIPFTLS TVSVCPIEEV APAIKRPMWF QLYVLRDRGF MRNALERAKA AGCSTLVFTV DMPTPGARYR DAHSGMSGPN AAMRRYLQAV THPQWAWDVG LNGRPHDLGN ISAYLGKPTG LEDYIGWLGN NFDPSISWKD LEWIRDFWDG PMVIKGILDP EDARDAVRFG ADGIVVSNHG GRQLDGVLSS ARALPAIADA VKGDIAILAD SGIRNGLDVV RMIALGADTV LLGRAFLYAL ATAGQAGVAN LLNLIEKEMK VAMTLTGAKS ISEITQDSLV QGLGKELPAA LAPMAKGNAA
- Purification
- purified by using conventional chromatography.
- Purity
- > 90% by SDS-PAGE
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1 MM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- L-Lactate Dehydrogenase (LLDD)
- Alternative Name
- L-lactate dehydrogenase/LLDD
- Background
- LldD, also known as L-lactate dehydrogenase, is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). Recombinant E. coli lldD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
- Molecular Weight
- 45.3 kDa (420aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_418062
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