TBCC Protein (AA 1-346) (His tag)
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- Target See all TBCC Proteins
- TBCC (Tubulin Folding Cofactor C (TBCC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-346
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TBCC protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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MESVSCS AAAVRTGDME SQRDLSLVPE RLQRREQERQ LEVERRKQKR QNQEVEKENS HFFVATFARE RAAVEELLER AESVERLEEA ASRLQGLQKL INDSVFFLAA YDLRQGQEAL ARLQAALAER RRGLQPKKRF AFKTRGKDAA SSTKVDAAPG IPPAVESIQD SPLPKKAEGD LGPSWVCGFS NLESQVLEKR ASELHQRDVL LTELSNCTVR LYGNPNTLRL TKAHSCKLLC GPVSTSVFLE DCSDCVLAVA CQQLRIHSTK DTRIFLQVTS RAIVEDCSGI QFAPYTWSYP EIDKDFESSG LDRSKNNWND VDDFNWLARD MASPNWSILP EEERNIQWD - Purification
- purified by chromatography
- Purity
- > 85% by SDS-PAGE
- Top Product
- Discover our top product TBCC Protein
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Tubulin Folding Cofactor C (TBCC) (AA 1-346) protein (His tag)
custom-made TBCC Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tubulin Folding Cofactor C (TBCC) (AA 1-341) protein (His tag)custom-made TBCC Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tubulin Folding Cofactor C (TBCC) (AA 1-346) protein (GST tag)TBCC Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Tubulin Folding Cofactor C (TBCC) protein (Myc-DYKDDDDK Tag)TBCC Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- TBCC (Tubulin Folding Cofactor C (TBCC))
- Alternative Name
- TBCC (TBCC Products)
- Background
- TBCC (tubulin folding cofactor C) belongs to the TBCC family. It is plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration. Cofactor C is one of four proteins (cofactors A, D, E and C) involved in the pathway leading to correctly folded b-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing b-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/b-tubulin complex, interaction with cofactor C then causes the release of b-tubulin polypeptides. Recombinant human TBCC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 41.7 kDa (369aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003183
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