MMP13 Protein (AA 104-471) (His tag)
Quick Overview for MMP13 Protein (AA 104-471) (His tag) (ABIN6387374)
Target
See all MMP13 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 104-471
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Purification tag / Conjugate
- This MMP13 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSYNVFPRT LKWSKMNLTY RIVNYTPDMT HSEVEKAFKK AFKVWSDVTP LNFTRLHDGI ADIMISFGIK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS HFMLPDDDVQ GIQSLYGPGD EDPNPKHPKT PDKCDPSLSL DAITSLRGET MIFKDRFFWR LHPQQVDAEL FLTKSFWPEL PNRIDAAYEH PSHDLIFIFR GRKFWALNGY DILEGYPKKI SELGLPKEVK KISAAVHFED TGKTLLFSGN QVWRYDDTNH IMDKDYPRLI EEDFPGIGDK VDAVYEKNGY IYFFNGPIQF EYSIWSNRIV RVMPANSILW C
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Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 266-471) protein (His-IF2DI Tag)
MMP13 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 104-274) protein (His tag)MMP13 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 20-148) protein (His tag)MMP13 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 20-471) protein (His tag)MMP13 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 20-471) protein (Fc Tag)MMP13 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 20-148) protein (Fc Tag)MMP13 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.15M NaCl, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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Alternative Name
- MMP13
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Background
- MMP13 of the matrix metalloproteinase (MMP) family is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Recombinant human MMP13 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 44.7 kDa (391aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002418
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UniProt
- P45452
Target
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