Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag)

Catalog No. ABIN6387821

Recombinant Lactate Dehydrogenase A protein expressed in Baculovirus infected Insect Cells.

Quick Overview for Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag) (ABIN6387821)

Target: Lactate Dehydrogenase A (LDHA)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-332

Purification tag / Conjugate: This Lactate Dehydrogenase A protein is labelled with His tag.

Sequence: MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 1  mM DTT, 20 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: Lactate Dehydrogenase A/LDHA

Background: Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 37.5 kDa (340aa)

NCBI Accession: NP_058721

UniProt: P04642

Pathways: Warburg Effect