GDA Protein (AA 1-454) (His tag)
Quick Overview for GDA Protein (AA 1-454) (His tag) (ABIN6387879)
Target
See all GDA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-454
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Purification tag / Conjugate
- This GDA protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV
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Biological Activity Comment
- Specific activity is >2,000pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
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Guanine Deaminase (GDA) (AA 1-454) protein (His tag)
custom-made GDA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)custom-made GDA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Guanine Deaminase (GDA) (AA 1-454) protein (GST tag)GDA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Guanine Deaminase (GDA) (AA 1-454) (Active) protein (His tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, EAA Active
Guanine Deaminase (GDA) (AA 1-454) (Active) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS, EAA Active
Guanine Deaminase (GDA) (AA 1-454) protein (His tag)GDA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Guanine Deaminase (GDA) proteinGDA Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GDA (Guanine Deaminase (GDA))
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Alternative Name
- Guanine deaminase/GDA
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Background
- GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 53 kDa (477aa)
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NCBI Accession
- NP_004284
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UniProt
- Q9Y2T3
Target
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