HPRT1 Protein (AA 1-218) (His tag)
Quick Overview for HPRT1 Protein (AA 1-218) (His tag) (ABIN6387916)
Target
See all HPRT1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-218
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Purification tag / Conjugate
- This HPRT1 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
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Biological Activity Comment
- Specific activity is > 15unit/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate(GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.
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Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 1-218) protein (GST tag)
HPRT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) (AA 1-218) protein (His tag)HPRT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) protein (Myc-DYKDDDDK Tag)HPRT1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing, 20 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))
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Alternative Name
- HPRT
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Background
- Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 26.7 kDa (238aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000185
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UniProt
- P00492
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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