PGP Protein (AA 1-321) (His tag)
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- Target See all PGP Proteins
- PGP (Phosphoglycolate Phosphatase (PGP))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-321
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PGP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG
- Purity
- > 95% by SDS-PAGE
- Biological Activity Comment
- Specific activity is > 3,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product PGP Protein
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Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)
custom-made PGP Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)custom-made PGP Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)PGP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycolate Phosphatase (PGP) protein (Myc-DYKDDDDK Tag)PGP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- PGP (Phosphoglycolate Phosphatase (PGP))
- Alternative Name
- PGP (PGP Products)
- Background
- Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.5 kDa (345aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001035830
- UniProt
- A6NDG6
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