UCHL1 Protein (AA 1-223) (His tag)
Quick Overview for UCHL1 Protein (AA 1-223) (His tag) (ABIN6388012)
Target
See all UCHL1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-223
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Purification tag / Conjugate
- This UCHL1 protein is labelled with His tag.
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Sequence
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MQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA -
Biological Activity Comment
- Specific activity is > 70pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C
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Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 1-220) protein (His tag)
UCHL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 1-223) protein (His tag)UCHL1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) protein (Fc Tag)UCHL1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 1-223) protein (GST tag)UCHL1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (Active) protein (His tag)UCHL1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 97 % as determined by SDS-PAGE Active
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (AA 1-223) proteinUCHL1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) (Active) protein (His tag)UCHL1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 97 % as determined by SDS-PAGE Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Liquid. In Phosphate buffered saline containing 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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Alternative Name
- PGP9.5/UCHL1
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Background
- Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 27.2 kDa (246aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_035800
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UniProt
- Q9R0P9
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Pathways
- Feeding Behaviour
Target
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